Genetic interaction analysis comes to the diploid human pathogen Candida albicans

[摘要] In the last 15 years, mycologists studying human fungal pathogens have seen great improvements in the methods for manipulating the genomes of these organisms. One result of these improvements is that large-scale deletion sets for Candida albicans [1], C. glabrata [2], Cryptococcus neoformans [3], and Aspergillus fumigatus [4] are available to the communities. This has greatly improved the depth and breadth of single gene–based genetic profiling of these important human pathogens. To date, however, these methods and resources have not been applied to the logical next step: functional genetic interaction analysis using strains with multiple mutations. The power of this approach has been most dramatically demonstrated in the model organism Saccharomyces cerevisiae based on the systematic approaches pioneered by Andrews, Boone, and others [5]. Although genetic networks have been constructed for important phenotypes in pathogenic fungi based on single gene analysis, expression profiling, and chromatin immunoprecipitation, the functional significance of such interactions have rarely been tested with double mutants.

[学科分类] 土木及结构工程学