Successful delivery following intracytoplasmic sperm injection with calcium ionophore A23187 oocyte activation in a partially globozoospermic patient

[摘要] Background: Systemic lupus erythematosus (SLE) is one of the pregnancy complications which cause an increase in fetal and maternal com­p­lications which are mainly caused by pla­cen­tal damage due to chronic inflammation and apoptosis. The rate of apoptosis can be assessed by the high expression of caspase-3 which is the ma­jo­rexecutioner caspases of apoptosis. The use of Mesenchymal Secretome Stem Cell for SLE the­rapy has an anti-inflammatory and anti-apo­p­to­tic role. This study aimed to examine the effect of mesenchymal secretome stem cell therapy to­ward caspase-3 expression in pregnant mice with pris­tan-induced lupus model. Subjects and Method: This study was a ran­do­mized controlled trial. This study was con­ducted at the Prodia ProStem Laboratory, La­bo­ra­tory of the Faculty of Veterinary Medicine of Airlangga University, and Anatomical Pathology Laboratory, Faculty of Medicine, Airlangga Uni­ver­sity. A total of 14 healthy BALB/C female mice strain aged 6-8 weeks, with a bodyweight of 20-30 grams, were divided into two groups, (1) 7 preg­nant mice who received pristan therapy only, and (2) 7 pregnant mice who received pristan the­rapy and mesenchymal secretome stem cell. The dependent variable was the caspase-3 exp­res­sion. The independent variable was the ad­mi­ni­s­tration of mesenchymal secretome stem cell the­rapy. The caspase-3 expression was measured by an immunoreactive score. The data were ana­ly­zed using independent t-test. Results: After giving mesenchymal secretome stem cell therapy, the mean of the caspase-3 exp­res­sion in the treatment group (Mean=1.86; SD­=­0.66) was lower than the control group (Me­an­=­3.­30; SD = 1.42), with p=0.031. Conclusion: Mesenchymal secretome stem cell the­rapy is effective for reducing caspase-3 exp­res­sion in the placenta of pregnant mice with pris­tan-induced lupus model.

[学科分类] 工业工程学

[关键词] Calcium ionophore A23187 Failed fertilization Globozoospermia ICSI Strontium chloride (SrCl2)