Lgr5 + intestinal stem cell sorting and organoid culture

[摘要] Intestinal epithelial stem cells (IESCs) are one of the most rapidly self‐renewing and proliferating adult stem cells. The IESCs reside at the bottom of intestinal and colonic crypts, giving rise to all intestinal epithelial lineages and maintaining intestinal epithe‐ lial replenishment. The technique of three‐dimensional culture based upon intestinal stem cell biology has been recently developed to study gastrointestinal development and disease pathogenesis. Here, we summarize the techniques used to isolate Lgr5‐ positive IESCs to form the enteroids from intestine or colonoids from colon, and pre‐ sent the means to examine these organoid functions. This study will provide a simple and practical way for producing intestinal tissues in the laboratory.

[学科分类] 机械工程学

[关键词] colonoids;enteroids;Lgr5 intestinal stem cells;organoid culture