Histopathology Study of Alginate Microspheres Containing Ovalbumin on Liver and Kidney Following Oral Administration and Evaluation of Uptake by Peyer’s Plaque

[摘要] Objectives: The development of oral vaccine formulations has been widely investigated to overcome oral route problems. This research investigated the in vivo immune response of ovalbumin-alginate microspheres by uptake compared with a commercial oral vaccine product. Materials and Methods: Ovalbumin-loaded alginate microspheres were prepared using aerosolization. Ovalbumin antigen in vivo uptake was investigated in order to understand the distribution and uptake by Peyer’s plaque (PP) after oral administration using fluorescence microscopy. The histopathology of ovalbumin-alginate microspheres in the liver and kidney was also investigated. Results: The use of alginate microspheres to deliver vaccines could be a promising delivery system for the development of oral vaccines because uptake by PP is an essential step in oral vaccination. Conclusion: Fluorescence visualization revealed the uptake of ovalbumin-loaded alginate microspheres with and without lyoprotectant maltodextrin by PP was equal to the oral vaccine product and no liver or kidney damage was found.

[关键词] Vaccine delivery;microspheres;histopathology