Development of LC Method for Estimation of Diethyl Carbamazine Citrate and Chlorpheniramine Maleate in Combined Dosage Form

[摘要] Liquid chromatographic method was developed for the quantitative determination of diethyl carbamazine citrate and chlorpheniramine maleate in combined dosage form. A Sunfre C18, 5 (im (250 mmx 4.6 mm i.d.) particle size column with mobile phase containing water: methanol: 10% triethyl amine (10:90:0.1, v/v; pH 5.5) was used. The fow rate was 1.0 mL/min and effuents were monitored at 225 nm. The retention times of diethyl carbamazine citrate and chlorpheniramine maleate were 3.4 min and 5.2 min, respectively. The linearity for diethyl carbamazine citrate and chlorpheniramine maleate were in the range of 1-100 (ig/mL and 0.1-20 (ig/mL, respectively. The proposed method was validated with respect to linearity, accuracy precision, specifcity and robustness. The method was successfully applied to the estimation of diethyl carbamazine citrate and chlorpheniramine maleate in combined dosage form.

[关键词] Diethyl carbamazine citrate;Chlorpheniramine maleate;Liquid chromatography;Validation