Detection of fimC gene as fingerprinting for Salmonella typhimurium isolates by using Polymerase Chain Reaction

[摘要] A total of 480 fecal samples were collected from children (less than 3 years old) , of both sexes suffering from diarrhea who admitted to The Teaching Hospital of Maternity and Pediatrics in Al- Diwaniya governorate, Salmonella spp. were isolated and identified using bacterial culturing on selective media, in addition to, biochemical and Mini API 20E and serotyping by monovalent antisera. Polymerase chain reaction (PCR) was used to detect fimC gene encoding for biosynthesis of fimC of Salmonella typhimurium. The results revealed that the rate of Salmonella isolates in fecal samples of patients were (38/480) 7.9% using cultural and Mini API20 E, The results of serotyping revealed that isolates there were 34 belong to Salmonella spp. Of these isolates 30 belong to S . typhimurium, while the remaining belong to S. enteritidis ( 2 isolates) and S. meunchen (2 isolates), when the PCR technique was used to detect the presence of fimC gene, 32 Salmonella isolates were belong to S. typhimurium appeared to contain this gene . The results of this study revealed that the PCR technique had a high specifity (100%) in detection of S. typhimurium in comparison to serotyping.

[学科分类] 土木及结构工程学