Histopathological changes and immunosuppression induce by diazepam in mice

[摘要] In order to investigate the histological changes and immunosuppression effects of diazepam in mice, forty white mice of both sexes were divided into four groups equally. 1 st group was immunized twice with pasturella multocida (bacterines) with two weeks intervals. 2 nd group was immunized as in the 1 st group and at same time administrated orally with 0.6 mg\kg b.w of diazepam daily for 8 weeks. 3 rd group was administrated with diazepam as in the 2 nd group while 4th group was served as control negative group. Immunological examination revealed that the diazepam inducing depresses of the both arms of immune response, the cell mediated immunity and humeral immunity as comparing with vaccinated non-treatment animals. The pathological examination revealed that the diazepam induced large multiple granulomatous liver lesions consist form aggregation mononuclear cells particularly macrophages and lymphocytes. In addition, section of kidney showed marked inflammatory cells infiltration particularly mononuclear cells and neutrophils in the interstitial tissues was seen. In spleen there was congestion of blood vessels with mononuclear cells in their lumen and depletion of white pulp as well as proliferation of megakaryocytes, in addition to gliosis in the brain parenchyma was seen. The immunized animals showed mild pathological changes characterized by aggregation mononuclear cells around central vein in addition to proliferation of kupffer cells, Spleen show proliferation of lymphocytes in the periartiriolar sheath as well as protein aqueous materials deposition around white pulp. We concluded that diazepam induced Histological changes in the internal organs of mice and stimulated the immune response diminished its toxic effects.

[学科分类] 土木及结构工程学

[关键词] Diazepam;Histological;Immune suppression