Combination of Curcuma (Curcuma xanthorriza Roxb) and Awar-awar (Ficus septica Burm.F.) Ethanolic Extracts Enhance Doxorubicin to Modulate Cell Cycle Progression of T47D Cells

[摘要] One of the efforts to cure breast cancer is a combination of the chemotherapeutic agent with medicinal plants. This study was conducted to examine the activity of the combination between doxorubicin, curcuma rhizome ( Curcuma xanthorriza Roxb.) ethanolic extract (CEE), and awar-awar leaves ( Ficus septica Burm.f.) ethanolic extract (AAE) in inducing apoptosis and modulating the cell cycle progression in breast cancer T47D cells. The combination activity was performed using three series of concentration, 1/3; 1/6 and 1/12 of IC 50 , The combination index (CI) of doxorubicin, CEE and AEE was determined under MTT assay. The result showed that the combination of 10 µM, 5 µg/ml, 1 µg/ml concentrations of  doxorubicin, CEE and AEE respectively result in synergistic effect with CI values less than 1. The treatment exhibited the cell accumulation in S phase (27.7%) against T47D breast cancer cells confirmed through cell cycle examination by flow cytometry. These results provided the evidence that CEE and the AEE can be developed as co-chemotherapeutic agents combined with doxorubicin to improve the effectiveness of breast cancer treatment.

[学科分类] 药学

[关键词] Curcuma xanthorriza Roxb.;Ficus septica Burm.f.;doxorubicin;cell cycle.