Single Cell Omics Approach: A Paradigm Shift in Diagnosis and Therapy of Cancer

[摘要] Relentless progress in molecular technologies over the decades has now solved the puzzle of carcinogenesis that it is heterogeneous in nature. Heterogeneity in cancer is primarily linked to the genetic alternations, as it is wellknown fact that random mutation frequency in human cancer cells is several fold greater than in nearby normal cells. So, it may translate abnormal protein qualitatively or may generate several copy of the same protein in quantities, as per basic mechanisms of central dogma of biology. But, with progression in cutting-edge technologies, now the complexity is unlocked beyond the simple mutation/aberration, now various unknown drivers of cancer have been explored through whole genome analysis. Thus, DNA copy number variation (CNV), methylation analysis, histonemodification, non-coding RNA expression, transcriptional profiling and splicing aberrations have been evident, which are significant contributor in complexity and heterogeneity of the cancer.