Assessment of Toxicity, Pro-Inflammatory Cytokines and Cytoprotective Potential of the Phytosaponin in Isolated Rat Pancreatic Islets

[摘要] The objective of this study was to evaluate the in-depth toxicity, assessment of pro-inflammatory and cytoprotective activity of phytosaponin. Saponin was isolated from methanolic extract of fruits of Momordica dioica and purification was achieved through the fractionation method of TLC. Toxicities were done as per OECD guidelines viz., In vivo micronucleus test (474), In vitro micronucleus test (487), In vitro chromosomal aberration test (473) in Neuroblastoma cells (NB41A3) lines and Ames test (471). Saponin Momordica dioica was screened for production of pro-inflammatory cytokines viz., IL-1β, IL-6 and TNF-α was determined by ELISA against high glucose-induced, cytoprotective activity like iNOS determined by western blot of against STZ-induced beta cell destruction in pancreatic isolated islets of rat. It was found that phytosaponin does not possess significant toxicities. Isolated pancreatic islet cells treated with high glucose produced pro-inflammatory cytokines which were reduced upon treatment with phytosaponin as compared to the high glucose control group. In similarly, mediator of inflammation like iNOS was assessed and analysis showed SMD inhibited moderately in STZ stimulated pancreatic islet cells. The results concluded, that phytosaponin possesses moderate to good cytoprotective nature and proves itself to be a potential candidate against diabetes The objective of this study was to evaluate the in-depth toxicity, assessment of pro-inflammatory and cytoprotective activity of phytosaponin. Saponin was isolated from methanolic extract of fruits of Momordica dioica and purification was achieved through the fractionation method of TLC. Toxicities were done as per OECD guidelines viz., In vivo micronucleus test (474), In vitro micronucleus test (487), In vitro chromosomal aberration test (473) in Neuroblastoma cells (NB41A3) lines and Ames test (471). Saponin Momordica dioica was screened for production of pro-inflammatory cytokines viz., IL-1β, IL-6 and TNF-α was determined by ELISA against high glucose-induced, cytoprotective activity like iNOS determined by western blot of against STZ-induced beta cell destruction in pancreatic isolated islets of rat. It was found that phytosaponin does not possess significant toxicities. Isolated pancreatic islet cells treated with high glucose produced pro-inflammatory cytokines which were reduced upon treatment with phytosaponin as compared to the high glucose control group. In similarly, mediator of inflammation like iNOS was assessed and analysis showed SMD inhibited moderately in STZ stimulated pancreatic islet cells. The results concluded, that phytosaponin possesses moderate to good cytoprotective nature and proves itself to be a potential candidate against diabetes.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] Saponin Momordica dioica;toxicity;pro-inflammatory cytokines;High glucose;STZ and cytoprotective