Investigations on Screening of Endophytic Fungal Fractions of Andrographis Paniculata (Burm. F.) Wall Nees. Leaves in Paracetamol and Ethanol Induced Hepatotoxicity

[摘要] To investigate the hepatoprotective effect of A4EA and A4nB extracts, prepared from the endophytic fungi Aspergillus tubingensis strain Cs/7/2 isolated from leaves of Andrographis paniculata on paracetamol and ethanol induced hepatotoxicity. APLF-4 was isolated from Andrographis paniculata leaves by Standard methods to get ethyl acetate (A4EA) and n butanol (A4nB) extracts. A4EA and A4nB were screened for hepatoprotective activity against paracetamol and ethanol induced hepatotoxicity at 50 mg/kg and 100 mg/kg p.o. in rats. Paracetamol and ethanol (50 &100 mg/kg) administration significantly elevated the levels of liver marker enzymes as compared to normal control group. A marked decrease in the levels of superoxide dismutase (SOD) and catalase (CAT) was also observed. Administration of A4EA and A4nB (50 &100 mg/kg) significantly raised the actions of paracetamol and ethanol by decreasing the levels of liver enzymes (***p<0.001), also increased the concentration of SOD and CAT (***p<0.001) and decreased lipid peroxidation (LPO). APLF-4 was isolated from the leaves of Andrographis paniculata and was identified as Aspergillus tubingensis strain Cs/7/2 by PCR sequential analysis and phylogenetic studies. A4EA and A4nB showed significant hepatoprotective activity in paracetamol and ethanol induced models. To investigate the hepatoprotective effect of A4EA and A4nB extracts, prepared from the endophytic fungi Aspergillus tubingensis strain Cs/7/2 isolated from leaves of Andrographis paniculata on paracetamol and ethanol induced hepatotoxicity. APLF-4 was isolated from Andrographis paniculata leaves by Standard methods to get ethyl acetate (A4EA) and n butanol (A4nB) extracts. A4EA and A4nB were screened for hepatoprotective activity against paracetamol and ethanol induced hepatotoxicity at 50 mg/kg and 100 mg/kg p.o. in rats. Paracetamol and ethanol (50 &100 mg/kg) administration significantly elevated the levels of liver marker enzymes as compared to normal control group. A marked decrease in the levels of superoxide dismutase (SOD) and catalase (CAT) was also observed. Administration of A4EA and A4nB (50 &100 mg/kg) significantly raised the actions of paracetamol and ethanol by decreasing the levels of liver enzymes (***p<0.001), also increased the concentration of SOD and CAT (***p<0.001) and decreased lipid peroxidation (LPO). APLF-4 was isolated from the leaves of Andrographis paniculata and was identified as Aspergillus tubingensis strain Cs/7/2 by PCR sequential analysis and phylogenetic studies. A4EA and A4nB showed significant hepatoprotective activity in paracetamol and ethanol induced models.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] Endophytic fungi;hepatoprotective;Andrographis paniculata;Aspergillus tubingensis strain Cs/7/2.