Isolation and Characterization of Oligomeric Procyanidins Rich PolyphenolFraction of Cinnamomum Zeylanicum Bark and it’s In Vitro Antidiabetic Studies

[摘要] Background: Cinnamomum zeylanicum Blume bark, a spice commonly used in the Indian household has been shown to have insulin-like biological activity.Objective: The present work was aimed at isolation and characterization of oligomeric procyanidins (OPCs) rich polyphenol fraction from C. zeylanicum bark and to carry out its in vitro anti-diabetic activity.Material and Methods: The polyphenol fraction from C. zeylanicum bark was afforded by extracting the bark with a series of solvents, such as 30% aqueous acetone, followed by hexane and butanol. The fraction was subjected to liquid chromatography–mass spectrometry (LCMS) analysis for characterization. From the LCMS data, presence of a few OPCs was confirmed. In vitro anti-diabetic activity was performed on the fraction of the bark by α-amylase inhibition and non-enzymatic hemoglobin glycosylation assays.Results and Conclusion: The fraction showed potential inhibition of glycosylation at half minimal inhibitory concentration (IC50) value of 102 µg/mL, while an α-amylase inhibition was observed to be poor at an IC50 value of 540 µg/mL. Thus, the fraction showed potential anti-diabetic effect by antioxidant mechanism.Keywords: C. zeylanicum, Polyphenols, Oligomeric procyanidins, α-amylase inhibition, Hemoglycosylation Background: Cinnamomum zeylanicum Blume bark, a spice commonly used in the Indian household has been shown to have insulin-like biological activity.Objective: The present work was aimed at isolation and characterization of oligomeric procyanidins (OPCs) rich polyphenol fraction from C. zeylanicum bark and to carry out its in vitro anti-diabetic activity.Material and Methods: The polyphenol fraction from C. zeylanicum bark was afforded by extracting the bark with a series of solvents, such as 30% aqueous acetone, followed by hexane and butanol. The fraction was subjected to liquid chromatography–mass spectrometry (LCMS) analysis for characterization. From the LCMS data, presence of a few OPCs was confirmed. In vitro anti-diabetic activity was performed on the fraction of the bark by α-amylase inhibition and non-enzymatic hemoglobin glycosylation assays.Results and Conclusion: The fraction showed potential inhibition of glycosylation at half minimal inhibitory concentration (IC50) value of 102 µg/mL, while an α-amylase inhibition was observed to be poor at an IC50 value of 540 µg/mL. Thus, the fraction showed potential anti-diabetic effect by antioxidant mechanism.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] C. zeylanicum;Polyphenols;Oligomeric procyanidins;α-amylase inhibition;Hemoglycosylation