Preparation, Characterization and Screening of Silver Nanoparticles Using Phenolic Rich Fractions of Amaranthus Gangeticus L. for its in-vitro Anti-Oxidant, Anti-Diabetic and Anti-Cancer Activities.

[摘要] Background : Nowadays, green nanotechnology-based approaches using plants have been acknowledged as harmless to the ecosystem and savvy approach with different biomedical applications.Aim: In the current study, we prepared silver nanoparticles (AgNPs) in combination with phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. (Leaves) using a suitable eco-friendly green-synthesis way to assess its in-vitro anti-oxidative, anti-diabetic and anti-cancer potential against HeLa cells.Methods: Plant mediated AgNPs were synthesized using phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. and characterization was done by UV–visible spectroscopy, X-ray diffraction (XRD), Fourier Transform infrared (FT-IR) spectroscopy, Scanning Electronic Microscopy (SEM) and TEM analysis. With the developing use of AgNPs in biomedical viewpoints, the synthesized AgNPs were assessed for its pharmacological studies.Results: The synthesized AgNPs have been confirmed by change of color which was identified by UV–visible spectroscopy (at 450 nm) due to surface plasmon resonance. The crystalline property of AgNPs was identified by its XRD pattern. Using FTIR, it was confirmed that the phenolic functional group present in plant extract was responsible for the reduction of silver ion and the stabilization of AgNPs. The morphology of AgNPs were studied by SEM and TEM analysis. The particles were monodispersed, white and spherical ranging from 10200 nm. With the developing use of AgNPs in biomedical viewpoint, the synthesized AgNPs were assessed for their in-vitro anti-oxidative, anti-diabetic and anti-cancer potential against HeLa cells. The results showed that the synthesized AgNPs exhibited good anti-diabetic, anti-cancer activity. It was also observed to exhibit potent anti-oxidant activity. Conclusions: From the present study, the synthesized AgNPs using Amaranthus gangeticus L.  were found to be very potent to treat the major diseases like cancer and diabetes. Background : Nowadays, green nanotechnology-based approaches using plants have been acknowledged as harmless to the ecosystem and savvy approach with different biomedical applications.Aim: In the current study, we prepared silver nanoparticles (AgNPs) in combination with phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. (Leaves) using a suitable eco-friendly green-synthesis way to assess its in-vitro anti-oxidative, anti-diabetic and anti-cancer potential against HeLa cells.Methods: Plant mediated AgNPs were synthesized using phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. and characterization was done by UV–visible spectroscopy, X-ray diffraction (XRD), Fourier Transform infrared (FT-IR) spectroscopy, Scanning Electronic Microscopy (SEM) and TEM analysis. With the developing use of AgNPs in biomedical viewpoints, the synthesized AgNPs were assessed for its pharmacological studies.Results: The synthesized AgNPs have been confirmed by change of color which was identified by UV–visible spectroscopy (at 450 nm) due to surface plasmon resonance. The crystalline property of AgNPs was identified by its XRD pattern. Using FTIR, it was confirmed that the phenolic functional group present in plant extract was responsible for the reduction of silver ion and the stabilization of AgNPs. The morphology of AgNPs were studied by SEM and TEM analysis. The particles were monodispersed, white and spherical ranging from 10200 nm. With the developing use of AgNPs in biomedical viewpoint, the synthesized AgNPs were assessed for their in-vitro anti-oxidative, anti-diabetic and anti-cancer potential against HeLa cells. The results showed that the synthesized AgNPs exhibited good anti-diabetic, anti-cancer activity. It was also observed to exhibit potent anti-oxidant activity. Conclusions: From the present study, the synthesized AgNPs using Amaranthus gangeticus L.  were found to be very potent to treat the major diseases like cancer and diabetes.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] Green nanotechnology;Anti-oxidant;Anti-diabetic;Anti-cancer