Hepatoprotective Potential of Endophytic Aspergillus Niger Strain A6 Fractions Isolated from Phyllanthus Amarus Schum and Thonn

[摘要] Aim: To isolate, characterize and screen for the in vitro antioxidant and hepatoprotective effects of P1EA and P1nB extracts from the endophytic fungi Aspergillus niger strain A6 (PALF-1) isolated from leaves of Phyllanthus amarus in paracetamol induced hepatotoxicity.Materials and methods: PALF-1 was isolated from Phyllanthus amarus leaves by standard procedures. Chloroform (P1C), ethyl acetate (P1EA) and n-butanol (P1nB) extracts were drawn up. P1C, P1EA and P1nB were screened for in vitro antioxidant activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical followed by reducing power assay. P1EA (50 and 100 mg/kg b. w.) and P1nB (50 and 100 mg/kg b. w.) were screened for hepatoprotective activity against paracetamol model in rats. High performance thin layer chromatography (HPTLC) profile of P1EA and P1nB was also studied. Characterization of PALF-1 was done by polymerase chain reaction (PCR) sequential analysis.Results: P1EA and P1nB found to scavenge DPPH, hydroxyl radicals and also showed reductive ability. In animal studies, administration of paracetamol (2 gm/kg b. w.) significantly increased the levels of liver biochemical marker enzymes in comparison to normal group. A marked decrease in the levels of superoxide dismutase (SOD) and catalase (CAT) was also observed. Administration of P1EA and P1nB (50 &100 mg/ kg) significantly reduced the actions of paracetamol by lowering the levels of serum biochemical parameters at various significance levels (***p<0.001; **p<0.01), increasing the levels of SOD and CAT (***p<0.001) and decreased lipid peroxidation (LPO). rDNA PCR sequencing analysis, phylogenetic and molecular studies of PALF-1 confirmed the endophytic fungus as Aspergillus niger strain A6. HPTLC finger printing of P1EA and PnB showed the presence of polyvalent compounds.Conclusion: P1EA and P1nB fractions of Aspergillus niger strain A6 showed significant antioxidant and hepatoprotective activity in paracetamol induced hepatotoxicity. This may be attributed to the antioxidant principles present in P1EA and P1nB which need to be explored. Aim: To isolate, characterize and screen for the in vitro antioxidant and hepatoprotective effects of P1EA and P1nB extracts from the endophytic fungi Aspergillus niger strain A6 (PALF-1) isolated from leaves of Phyllanthus amarus in paracetamol induced hepatotoxicity.Materials and methods: PALF-1 was isolated from Phyllanthus amarus leaves by standard procedures. Chloroform (P1C), ethyl acetate (P1EA) and n-butanol (P1nB) extracts were drawn up. P1C, P1EA and P1nB were screened for in vitro antioxidant activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical followed by reducing power assay. P1EA (50 and 100 mg/kg b. w.) and P1nB (50 and 100 mg/kg b. w.) were screened for hepatoprotective activity against paracetamol model in rats. High performance thin layer chromatography (HPTLC) profile of P1EA and P1nB was also studied. Characterization of PALF-1 was done by polymerase chain reaction (PCR) sequential analysis.Results: P1EA and P1nB found to scavenge DPPH, hydroxyl radicals and also showed reductive ability. In animal studies, administration of paracetamol (2 gm/kg b. w.) significantly increased the levels of liver biochemical marker enzymes in comparison to normal group. A marked decrease in the levels of superoxide dismutase (SOD) and catalase (CAT) was also observed. Administration of P1EA and P1nB (50 &100 mg/ kg) significantly reduced the actions of paracetamol by lowering the levels of serum biochemical parameters at various significance levels (***p<0.001; **p<0.01), increasing the levels of SOD and CAT (***p<0.001) and decreased lipid peroxidation (LPO). rDNA PCR sequencing analysis, phylogenetic and molecular studies of PALF-1 confirmed the endophytic fungus as Aspergillus niger strain A6. HPTLC finger printing of P1EA and PnB showed the presence of polyvalent compounds.Conclusion: P1EA and P1nB fractions of Aspergillus niger strain A6 showed significant antioxidant and hepatoprotective activity in paracetamol induced hepatotoxicity. This may be attributed to the antioxidant principles present in P1EA and P1nB which need to be explored.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] Endophytic fungus;Hepatoprotective;Paracetamol toxicity;Phyllanthus amarus;Aspergillusniger strain A6