Antioxidant and Antidiabetic Potential of Endophytic Fungal Fractions Recovered from Tinospora Cordifolia (Willd.) Leaves

[摘要] Background: Diabetes mellitus (DM) is a severe metabolic disorder caused by lack of insulin secretion. Endophytes are microorganisms having the potentiality in colonizing internal tissues of the plant. Previous research reported that they are potential sources of novel secondary metabolites which are useful in medicine and agriculture.Objective: In this study, an attempt was made to isolate, characterize and screen for endophytic fungi from the leaves of Tinospora cordifolia for in vitro antioxidant and antidiabetic activity.Materials and Methods: Isolation of endophytic fungi was accomplished by established method. Pure culturable endophytic fungi were identified and characterized by PCR sequential analysis and phylogenetic studies. Chloroform, ethyl acetate and n-butanol fractions of endophytic fungi were screened for in vitro free radical scavenging (2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power assay) and antidiabetic in α-amylase, α- glucosidase and aldose reductase inhibitory assays.Results: Two fungal endophytes were isolated (TCLF-1 and TCLF-2) from leaves of Tinospora cordifolia with ethyl acetate (T1EA, T1EA) and n-butanol (T2nB, T2nB). TCLF-1 and TCLF-2 fractions exhibited appreciable α-amylase, α- glucosidase and aldose reductase inhibitory activity with significant IC50 values when compared with acarbose. Molecular characterization and phylogenetic studies identified TCLF-1 and TCLF-2 fungi as Trichoderma longibrachiatum isolate TL10 and Aspergillus versicolor isolate BAB-6580 respectively.Conclusion: The results of the present study indicate that ethyl acetate and n-butanol fractions of TCLF-1 and TCLF-2 showed appreciable free radical scavenging and in vitro antidiabetic activity. Background: Diabetes mellitus (DM) is a severe metabolic disorder caused by lack of insulin secretion. Endophytes are microorganisms having the potentiality in colonizing internal tissues of the plant. Previous research reported that they are potential sources of novel secondary metabolites which are useful in medicine and agriculture.Objective: In this study, an attempt was made to isolate, characterize and screen for endophytic fungi from the leaves of Tinospora cordifolia for in vitro antioxidant and antidiabetic activity.Materials and Methods: Isolation of endophytic fungi was accomplished by established method. Pure culturable endophytic fungi were identified and characterized by PCR sequential analysis and phylogenetic studies. Chloroform, ethyl acetate and n-butanol fractions of endophytic fungi were screened for in vitro free radical scavenging (2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power assay) and antidiabetic in α-amylase, α- glucosidase and aldose reductase inhibitory assays.Results: Two fungal endophytes were isolated (TCLF-1 and TCLF-2) from leaves of Tinospora cordifolia with ethyl acetate (T1EA, T1EA) and n-butanol (T2nB, T2nB). TCLF-1 and TCLF-2 fractions exhibited appreciable α-amylase, α- glucosidase and aldose reductase inhibitory activity with significant IC50 values when compared with acarbose. Molecular characterization and phylogenetic studies identified TCLF-1 and TCLF-2 fungi as Trichoderma longibrachiatum isolate TL10 and Aspergillus versicolor isolate BAB-6580 respectively.Conclusion: The results of the present study indicate that ethyl acetate and n-butanol fractions of TCLF-1 and TCLF-2 showed appreciable free radical scavenging and in vitro antidiabetic activity.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] Tinospora cordifolia endophytes;Antidiabetic;Antioxidant;Trichoderma longibrachiatum;Aspergillus versicolor BAB-6580