Characterization of Capecitabine Loaded Stealth Liposomes by Nuclear Magnetic Resonance

[摘要] Background: Liposomes are lipid aggregates with a careful choice of lipid composition and process parameters. Liposomes are formulated by optimization of process parameters of liposomes to achieve desired bioavailability and to improve stability. Objectives: The main objective of the present research was to formulate colon targeting capecitabine loaded stealth liposomes, characterized by using Nuclear magnetic resonance (NMR) techniques to determine the fractions of the drug, capecitabine (CAP), encapsulated in the liposomal suspension.Methods: Liposome properties were evaluated by NMR. 31P-NMR (31Phosphorus NMR) in combination with the use of chemical shift reagents were used for the identification of lamellarity of liposomes which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds.Results: The results suggested that capecitabine remained intact during the loading process, and the liposomal drug was present in a soluble form. Conclusion: 31P-NMR was found to be an effective method to study the lamellarity of liposomes and to determine the distributed fractions of encapsulated capecitabine. Background: Liposomes are lipid aggregates with a careful choice of lipid composition and process parameters. Liposomes are formulated by optimization of process parameters of liposomes to achieve desired bioavailability and to improve stability. Objectives: The main objective of the present research was to formulate colon targeting capecitabine loaded stealth liposomes, characterized by using Nuclear magnetic resonance (NMR) techniques to determine the fractions of the drug, capecitabine (CAP), encapsulated in the liposomal suspension.Methods: Liposome properties were evaluated by NMR. 31P-NMR (31Phosphorus NMR) in combination with the use of chemical shift reagents were used for the identification of lamellarity of liposomes which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds.Results: The results suggested that capecitabine remained intact during the loading process, and the liposomal drug was present in a soluble form. Conclusion: 31P-NMR was found to be an effective method to study the lamellarity of liposomes and to determine the distributed fractions of encapsulated capecitabine.

[学科分类] 药学、药理学、毒理学（综合）

[关键词] Capecitabine;Liposomes;Nuclear magnetic resonance;Signal splitting;31P NMR;Lamellarity