Chrysophanol inhibits the progression of gastric cancer by activating nod-like receptor protein-3

[摘要] Aim: Gastric cancer (GC) is one of the most common malignant tumors. Chrysophanol has been reported topossess antitumor effects on a variety of cancers; however, its role in GC remains unclear. This study aimed to investigatethe effects of chrysophanol on the proliferation, pyroptosis, migration, and invasion of GC cells. Methods: Human GCcell lines MKN 28 and AGS cells were treated with different concentrations of chrysophanol, then cell proliferation,migration, invasion and pyroptosis were determined by CCK-8, colony-forming assay, wound healing assay, Transwellassay, and flow cytometry. Cell migration and invasion were reassessed in these transfected cells following thetransfection of nod-like receptor protein-3 (NLRP3) siRNA in MKN 28 and AGS cells. To examine the downstreamsignaling pathway of the NLRP3 signaling pathway, NLRP3, caspase-1, gasdermin-D, interleukin (IL)-1β, and IL-18were detected by quantitative real-time-polymerase chain reaction or western blotting. Results: Chrysophanolinhibited the proliferation of GC cells, caused pyroptosis, inhibited cell migration and invasion, and increased theexpression of NLRP3 inflammasomes in GC cells. Knockdown of NLRP3 inhibited the effects of chrysophanol onproliferation, pyroptosis, migration, and invasion of GC cells. Chrysophanol plays an anticancer role by enhancingNLRP3. Conclusions: Chrysophanol exerts anti-neoplastic effects in vitro in GC cells by modulating NLRP3, thushighlighting its therapeutic potential in GC.

[学科分类] 仪器

[关键词] Chrysophanol;Gastric cancer;NLRP3 inflammasome;Caspase-1;Pyroptosis