In vitro study of emodin-induced nephrotoxicity in human renal glomerular endothelial cells on a microfluidic chip"> In vitro study of emodin-induced nephrotoxicity in human renal glomerular endothelial cells on a microfluidic chip

[摘要] Emodin is an effective component of rhubarb with positive pharmacological effects on human health. However, it isalso toxic to different cells or tissues to varying degrees. The effects of emodin on glomerular endothelial cells (GECs) remain tobe tested, and the documented works were always performed in vitro and hardly reflect the real physiological situation. Tostudy the effects of emodin on GECs in a biomimetic environment, we utilized a microfluidic chip to assess thephysiological reaction of human renal glomerular endothelial cells to various concentrations of emodin in this work. Theresults showed that emodin caused cytotoxicity, impaired glomerular filtration barrier integrity to macromolecules, andincreased barrier permeability in a dose-dependent manner. With the increase in emodin concentration, the concentrationof the pro-inflammatory cytokine tumor necrosis factor-α, interleukin (IL)-6, transforming growth factor-β1, andmonocyte chemoattractant protein (MCP-1) increased while the production of inflammatory cytokine IL-6 first increasedand then decreased with the increase in emodin concentration. Our findings shed new light on emodin-inducednephrotoxicity and provide insights for the application of microfluidic chip devices to reveal drug-cell interactions.

[学科分类] 仪器

[关键词] Emodin;Nephrotoxicity;HRGECs;Microfluidic chip