ScCaM gene negatively regulates salinity and drought stress responses in transgenic Arabidopsis thaliana ">Overexpression of a sugarcane ScCaM gene negatively regulates salinity and drought stress responses in transgenic Arabidopsis thaliana

[摘要] Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study,the effects of a sugarcane ScCaM gene (NCBI accession number: GQ246454) on drought and salt stress tolerance intransgenic Arabidopsis thaliana and Escherichia coli cells were evaluated. The results demonstrated a significantnegative role of ScCaM in the drought and salt stress tolerance of transgenic lines of A. thaliana, as indicated by thephenotypes. In addition, the expression of AtP5CS and AtRD29A, two genes tightly related to stress resistance, wassignificantly lower in the overexpression lines than in the wild type. The growth of E. coli BL21 cells expressingScCaM showed weaker tolerance under mannitol and NaCl stress. Taken together, this study revealed that the ScCaMgene plays a negative regulatory role in both mannitol and NaCl stresses, and it possibly exerts protective mechanismscommon in both prokaryotes and eukaryotes under stress conditions.

[学科分类] 仪器

[关键词] Calmodulin (CaM);Signal transduction;Drought;Salt stress;Sugarcane