Quercetin induced HepG2 cells apoptosis through ATM/JNK/STAT3 signaling pathways

[摘要] Liver cancer is the seventh most common malignant tumor in the world and is the second highest cause of deathdue to cancer. Quercetin, a flavonoid with low toxicity, widely exists in various fruits and vegetables. It has the potential to bea therapeutic agent against various cancers. This study aimed to demonstrate the anti-tumor effect of quercetin on HepG2cells. Quercetin suppressed the HepG2 cell proliferation in a dose-dependent manner in cell viability assay. Induction of cellapoptosis was confirmed by apoptotic cells population (sub-G1 peak) detected by flow cytometer. A decrease inmitochondrial membrane potential and caspase-3 activation were also demonstrated in this study. Furthermore,quercetin induced HepG2 cell apoptosis through ROS-mediated phosphorylated ataxia-telangiectasia mutated, c-Jun Nterminal kinases, signal transducer, and activator of transcription 3 (STAT-3), and Bax signaling pathways. These resultssuggest that quercetin has the potential to become an effective drug against the tumor.

[学科分类] 仪器

[关键词] Quercetin;Caspase-3;Reactive oxygen species;Apoptosi