in vivo lineage-tracing approaches: A new opportunity for stem cell biology">Synergy of single-cell sequencing analyses and in vivo lineage-tracing approaches: A new opportunity for stem cell biology

[摘要] Single-cell sequencing technologies have rapidly progressed in recent years, and been applied to characterize stem cells in a number of organs. Somatic (postnatal) stem cells are generally identified using combinations of cell surface markers and transcription factors. However, it has been challenging to define micro-heterogeneity within “stem cell” populations, each of which stands at a different level of differentiation. As stem cells become defined at a single-cell level, their differentiation path becomes clearly defined. Here, this viewpoint discusses the potential synergy of single-cell sequencing analyses with in vivo lineage-tracing approaches, with an emphasis on practical considerations in stem cell biology.

[学科分类] 仪器

[关键词] Single-cell sequencing;in vivo lineage-lineage;skeletal stem cells (SSCs);mesenchymal stem cells (MSCs);bone marrow stromal cells (BMSCs)