+ CD38 - subpopulation without CD123 and CD44 is responsible for LSC and correlated with imbalance of immune cell subsets in AML">CD34 + CD38 - subpopulation without CD123 and CD44 is responsible for LSC and correlated with imbalance of immune cell subsets in AML

[摘要] Acute myeloid leukemia (AML) is regarded as a stem cell disease. However, no one unique marker is expressedon leukemia stem cells (LSC) but not on leukemic blasts nor normal hematopoietic stem cells (HSC). CD34+CD38- withor without CD123 or CD44 subpopulations are immunophenotypically defined as putative LSC fractions in AML.Nevertheless, markers that can be effectively and simply held responsible for the intrinsical heterogeneity of LSC isstill unclear. In the present study, we examined the frequency of three different LSC subtypes (CD34+CD38-,CD34+CD38-CD123+, CD34+CD38-CD44+) in AML at diagnosis. We then validated their prognostic significance onthe relevance of spectral features for diagnostic stratification, immune status, induction therapy response, treatmenteffect maintenance, and long-term survival. In our findings, high proportions of the above three different LSCsubtypes were all significantly characterized with low complete remission (CR) rate, high relapse/refractory rate, pooroverall survival (OS), frequent FLT3-ITD mutation, the high level of regulatory T cells (Treg) and monocyticmyeloid-derived suppressor cells (M-MDSC). However, there was no significant statistical difference in all kinds ofother clinical performance among the three different LSC groups. It was demonstrated that CD34+CD38-subpopulation without CD123 and CD44 might be held responsible for LSC and correlated with an imbalance ofimmune cell subsets in AML.

[学科分类] 仪器

[关键词] Acute myeloid leukemia;Leukemia stem cells;CD123;CD44;Immune cell subsets