25-35 ">Neural stem cell-conditioned medium upregulated the PCMT1 expression and inhibited the phosphorylation of MST1 in SH-SY5Y cells induced by Aβ 25-35

[摘要] A progressive neurodegenerative disease, Alzheimer’s disease (AD). Studies suggest that highly expressedprotein isoaspartate methyltransferase 1 (PCMT1) in brain tissue. In the current study, we explored the effects ofneural stem cell-conditioned medium (NSC-CDM) on the PCMT1/MST1 pathway to alleviate Aβ25-35-induceddamage in SH-SY5Y cells. Our data suggested that Aβ25-35 markedly inhibited cell viability. NSC-CDM or Neuralstem cell-complete medium (NSC-CPM) had a suppression effect on toxicity when treatment with Aβ25-35, with agreater effect observed with NSC-CDM. Aβ25-35 + NSC-CDM group exhibited an increase in PCMT1 expression.sh-PCMT1 markedly decreased cell proliferation and suppressed the protective role of NSC-CDM through theinduction of apoptosis and improved p-MST1 expression. Overexpression of PCMT1 reversed the Aβ25-35-induceddecrease in cell proliferation and apoptosis. In summary, our findings suggest that NSC-CDM corrects the Aβ25-35-induced damage to cells by improving PCMT1 expressions, which in turn reduces phosphorylation of MST1.

[学科分类] 仪器

[关键词] Neural stem cell conditioned medium;Protein isoaspartate methyltransferase 1;MST1;Amyloid β25-35;Apoptosis