Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells

[摘要] Sorafenib, a multikinase inhibitor used for the treatment of hepatocellular carcinoma, is limited by its low oralbioavailability. To overcome this drawback, we have developed novel camel milk casein-derived nanoparticles as a drugdelivery system. Camel milk casein is not only biocompatible on oral administration but is actually a dietary protein ofpharmaceutical relevance. Casein is used because of its amphiphilic nature, self-assembling property, ability to showsustained release, and capability of encapsulating both hydrophilic and hydrophobic drugs. In this study, camel milkcasein nanoparticles loaded with sorafenib were developed and characterized. Characterization of casein nanoparticleswas done by dynamic light scattering (DLS), zeta potential analysis, scanning light microscopy (SEM), and FTIR. Thedrug content in nanoparticle and drug-protein binding studies were conducted by UV spectroscopy. The cytotoxicityand cellular uptake efficiency studies were performed in HepG2 cell lines. It was observed that the cytotoxic effect ofsorafenib loaded camel milk casein nanoparticles was more than free sorafenib in HepG2 cells. This work suggestscamel milk casein as a suitable drug delivery molecule for sorafenib. In the future, it may also be used in enhancingthe efficacy and specific distribution of other water-insoluble anticancer drugs.

[学科分类] 仪器

[关键词] Camel milk derived casein nanoparticles;Sorafenib;UV spectroscopy;FTIR;HepG2 cells;Apoptosis