Identification of tumorigenic risk genes in human placenta-derived mesenchymal stem cells treated with 3-methylcholanthrene

[摘要] Mesenchymal stem cells (MSCs) capable of tumour topotaxis have been served as cellular vehicles to deliveranti-tumour agents. As cellular components of the tumour microenvironment, MSCs also affect tumour progression.However, the tumour transformation-related genes of MSCs remain unclear since either tumorigenic or tumoursuppressor effects within these cells have been researched. Hence, we aimed to identify potential biomarkers indicativeof tumorigenic risk by RNA-seq analysis of human placenta tissue-derived MSCs (hPTMSCs) exposed to thecarcinogenic agent, 3-methylcholanthrene (3-MC). Twenty-nine tumour transformation-related genes and threepluripotency-related genes were appraised as differentially expressed genes (DEGs) in hPTMSCs. Overexpression ofsfrp1 led to reduced cell viability, migration, and colony formation in A549. In contrast, the overexpression of ptgs2exerted the opposite effect. These results indicate that A549 cells with high ptgs2 expression but low sfrp1 expressionmay have a more potential tumorigenic capacity. Taken together, this study suggests that ptgs2 and sfrp1 may betumorigenic risk genes.

[学科分类] 仪器

[关键词] Human placenta-derived mesenchymal stem cells;Transcriptional profile;Tumorigenicity