Lycopus lucidus Turcz. supplementation on gut microflora and short chain fatty acid composition in Crj: CD-1 mice">Effect of Lycopus lucidus Turcz. supplementation on gut microflora and short chain fatty acid composition in Crj: CD-1 mice

[摘要] We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice. Inaddition, we evaluated the production of major cytokines (Interleukin-6 and -10) which are related to inflammationand fatty acid composition of several tissues. 16S ribosomal DNA sequencing-based microbiome taxonomic profilinganalysis was performed utilizing the EzBioCloud data base. Male mice fed on L. lucidus showed a significantlyreduced number of lactic acid bacteria and coliform in the feces compared with the control group (p < 0.05). 16SrDNA sequencing analysis of fecal samples showed that L. lucidus supplementation decreased the community ofharmful microflora (Enterobacteriaceae including Escherichia coli and Bacteroides sp.) in feces compared with thecontrol group (p < 0.05). There were no significant differences in mRNA expression of cytokine IL-6 and IL-10between the control and L. lucidus fed groups. The fecal fatty acid composition in the L. lucidus group hadpercentages of 4:0, 6:0, 8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the controlgroup. Our results showed that L. lucidus supplementation influenced gut environment by decreasing harmfulmicroflora and increased the percentages of several short fatty acids.

[学科分类] 仪器

[关键词] Lycopus lucidus Turcz;Fatty acid composition;Intestinal microflora;16S rDNA sequencing;Cytokines