Hsa_circ_0002137 stabled by LIN28B promotes osteosarcoma cell growth through the hsa-miR-1246/BCL2 axis

[摘要] Circular RNAs (circRNAs) are a novel class of non-coding RNA that have recently shown to have hugecapabilities in the regulation of gene expression at the posttranscriptional level. Growing evidence has indicated thatcircRNAs could serve as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) and suppressfunctions of targeted miRNAs. Osteosarcoma (OS) is the most common malignant primary bone cancer.Hsa_circ_0002137 is upregulated in OS. However, the role of hsa_circ_0002137 in OS remains unclear. Using miRNApull-down assay, we showed that cir_0002137 sponged hsa-miR-1246, and BCL2 apoptosis regulator (BCL2) mRNAwas a potential target of hsa-miR-1246 in human osteosarcoma (HOS) cells. Further, we found that hsa_cir_0002137could enhance the expression of BCL2 hsa-miR-1246 and promote HOS cell growth through sponging hsa-miR-1246.Moreover, RNA binding protein immunoprecip itation (RIP) assay revealed that lin-28 homolog B (LIN28B) proteinassociated with hsa_circ_0002137, and LIN28B could increase hsa_circ_0002137 stability and thus accelerate OS cellgrowth. Our work was the first to study the functions of hsa_circ_0002137, has-miR-1246 and LIN28B in OS, andthese results may provide novel therapeutic targets for OS treatment.

[学科分类] 仪器

[关键词] Osteosarcoma;hsa_circ_0002137;hsa-miR-1246;Proliferation;Apoptosis