Claroideoglomus etunicatum improved the growth and saline– alkaline tolerance of Potentilla anserina by altering physiological and biochemical properties"> Claroideoglomus etunicatum improved the growth and saline– alkaline tolerance of Potentilla anserina by altering physiological and biochemical properties

[摘要] To investigate the effects of arbuscular mycorrhizal (AM) fungi on the growth and saline–alkaline tolerance ofPotentilla anserina L., the seedlings were inoculated with Claroideoglomus etunicatum (W.N. Becker & Gerd.) C. Walker &A. Schüßler in pot cultivation. After 90 days of culture, saline–alkaline stress was induced with NaCl and NaHCO3solution according to the main salt components in saline–alkaline soils. Based on the physiological response of P. anserinato the stress in the preliminary experiment, the solution concentrations of 0 mmol/L, 75 mmol/L, 150 mmol/L, 225 mmol/Land 300 mmol/L were treated with stress for 10 days, respectively. The mycorrhizal colonization rate, mycorrhizaldependence, chlorophyll content, malondialdehyde content, antioxidant enzyme activities, osmoregulation substancescontent and water status were measured. The results showed that with the increase of NaCl and NaHCO3 stressconcentration, mycorrhizal colonization rate, colonization intensity, arbuscular abundance and vesicle abundancedecreased, and reached the lowest value at 300 mmol/L. Strong mycorrhizal dependence was observed after the symbiosiswith AM fungus, and the dependence was higher under NaHCO3 treatment. Under NaCl and NaHCO3 stress, inoculationwith AM fungus could increase chlorophyll content, decrease malondialdehyde content, increase activities of superoxidedismutase, peroxidase and catalase, increase contents of proline, soluble sugar and soluble protein, increase tissue relativewater content and decrease water saturation deficit. It was concluded that salt–alkali stress inhibited the colonization ofAM fungus, but the mycorrhiza still played a positive role in maintaining the normal growth of plants under salt–alkali stress.

[学科分类] 仪器

[关键词] Arbuscular mycorrhizal fungi;Colonization characteristics;Antioxidant system;Osmoregulation substances;Water status