Chromatographic Separation of Various Pharmacological Classes of Therapeutic Agents on Impregnated Thin Layer Silica Gel Plates

[摘要] Thin Layer Chromatography (TLC) is a useful liquid chromatographic technique use to separate and identify chemical substances inmixtures. Analytically, the technique has been used to identify and quantify therapeutic agents in pharmaceutical drug products andbiological fluids. Surfactants are organic compounds with at least one hydrophilic group and one hydrophobic group in the molecule. Buffersolutions are solutions of constant PH values. To investigate the degree of separation of various pharmacological classes of therapeuticagents which are co-administered (based on prescriptions) on surfactant and PH control-impregnated thin layer chromatographic plates. ThinLayer Chromatography (TLC) is the separation of chemical substances between two phases (stationary solid phase and mobile liquid solventphase) based on differences in solubility of chemical substances in the two phases [1]. Several studies have reported the chromatographicseparations of chemical substances on silica gel-impregnated thin layer chromatography with the primary aim of improving separation ofvarious classes of chemical substances. Studies [2,3] have reported the separation of aliphatic amines, sulfa drugs and phenolic compoundson metal salts or soap-impregnated silica gel plates. Furthermore, aromatic amines have been separated on surfactant-impregnated silica gelplates [4].

[学科分类] 药理学