Bioinformatics and Genetic Modification Methodologies for Generating Recombinant Biopharmaceutical Membrane Proteins in Phytoplankton

[摘要] In recent years, there has been a noticeable surge in interest in using microalgae as cell factories to generate biopharmaceuticalglycoproteins. Microalgae frequently create biopharmaceutical glycoproteins by post-translational protein N-glycosylation. The Nglycosylation process influences the physicochemical characteristics of biopharmaceutical glycoproteins such as folding, stability, andmolecular recognition. As a result, detailed study of the processes and routes of N-glycosylation in microalgae is required to betterunderstand the activity and function of biopharmaceutical glycoproteins. Using sophisticated bioinformatics approaches, researchersexamined entire genome databases of several microalgae for the presence and evolutionary connection of suspected GolgiGlycosyltransferases (GTs) and Glycosidases (GSs). The findings revealed that the existence and number of GTs, GSs, and mature N-glycanstructures differed depending on the organism. GTs and GSs might have complicated evolutionary links and various activities in differentcreatures since they have complex phylogenetic trees and conserved motif designs. The microalgal and human N-glycosylation processeswere quite different, suggesting that more work will be required to create humanized therapeutic glycoproteins in microalgae.

[学科分类] 药理学