An Improved Technique for Genotyping the ABCB1 Gene Variant of Exon 21

[摘要] The Multidrug Resistance protein (ABCB1, MDR1) is involved in the transport of xenobiotics and antiretroviral drugs. Some variants of the ABCB1T/A for the exon 21 doing only one PCR with the corresponding primers and the digestion of the PCR product with two restriction enzymes: BrsI to identify A allele and BseYI to differentiate between G or T. An improvement of this methodology was also described. The proposal technique here described is demonstrated to be very efficient, easy, fast, reproducible, and cost-effective.

[学科分类] 电力

[关键词] ABCB1 gene;Exon 21;gene variants;genotyping method;single nucleotide variants;restriction fragment length polymorphisms;PCR