Comparative evaluation of antimicrobial efficacy of oregano essential oil extract with four other intracanal medicaments against Enterococcus faecalis – An in vitro study

[摘要] Background:  The rationale of endodontic treatment is to eradicate the infection and prevent the microorganisms from infecting or re-infecting the root canal system (RCS). Gram-negative anaerobic rods, Gram-positive anaerobic cocci, Gram-positive anaerobic, and facultative rods are most commonly isolated from the RCS. Herbal compounds can be used as a promising alternative to conventional calcium hydroxide. The current study aimed to compare and evaluate the antimicrobial effect of four different intracanal medicaments against Enterococcus faecalis. Aim:  The objective of the current study is to compare and evaluate the antimicrobial effect of a new herbal intracanal medicament compared with the other four agents against E. faecalis. Materials and Methods:  A total of 100 single-rooted teeth were selected, root canal preparation was performed using a standardized protocol, inoculated E. faecalis, which were further divided into five groups each according to intracanal medicaments used. Group 1: Calcium Hydroxide-16 mg/ml of distilled water (n = 20), Group 2: Diclofenac–400 mg/ml of distilled water (n = 20), Group 3: Ibuprofen–400 mg/ml of distilled water (n = 20), Group 4: Omeprazole–2 mg/ml of distilled water (n = 20), and Group 5: Oregano essential oil–25 μg/ml of distilled water (n = 20). The antimicrobial assessment was performed on the 7th day, and dentine samples were collected to determine the number of colony-forming units. One-way analysis of variance and post hoc Tukey test was performed. Results:  Group 1 (calcium hydroxide) showed a statistically significant reduction of E. faecalis (P < 0.05), followed by group 5 (Oregano essential oil extract). Conclusion:  Calcium hydroxide showed a beneficial reduction of E. faecalis when compared to other tested agents.

[学科分类] 材料科学（综合）

[关键词] Calcium hydroxide;diclofenac;Enterococcus faecalis;ibuprofen;omeprazole;oregano essential oil