P2X7 receptor involved in antitumor activity of atractylenolide I in human cervical cancer cells

[摘要] Atractylenolide I (Atr-I) was found to sensitize a variety of human cancer cells in previous studies. Purinergic P2X7R playsimportant role in diferent cancers. However, whether Atr-I could generate antitumor activity in human cervical cancer cellsand P2X7R get involved in this efect remain unclear. In this study, Hela (HPV 18+) and SiHa (HPV 16+) cells were treatedwith diferent doses of Atr-I. The results indicated that agonist and antagonist of P2X7 receptors, BzATP and JNJ-47965567(JNJ), could suppress the proliferation of Hela and SiHa cells. Atr-I demonstrated a considerable antitumor efect in bothhuman cervical cancer cells in vitro. Atr-I combined with P2X7R agonist, BzATP, restored Atr-I-induced growth inhibitionin Hela cells but not in SiHa cells. However, the combinatorial treatment of P2X7R antagonist JNJ and Atr-I has an additiveefect on cell growth inhibition in SiHa cells rather than in Hela cells. It implied that P2X7R would get involved in the antihuman cervical cancer cells efect of Atr-I.

[学科分类] 分子生物学，细胞生物学和基因

[关键词] Atractylenolide I;P2X7Rs;Hela cells;SiHa cells;Human cervical cancer cells