Whitening and Anti-oxidative Activities of Chemical Components Extracted from Branches of Sorbus alnifolia

[摘要] In this study were evaluated the whitening and anti-oxidative activities from the extracts of Sorbus alnifoliabranches, and identified the chemical structures of the active ingredients. In the whitening tests using α-MSH stimulatedB16F10 melanoma cells, the 70% ethanol extract and n-butanol (n-BuOH) fractions concentration-dependently inhibited cellularmelanogenesis and intracellular tyrosinase activities without causing cell toxicity. The total polyphenol content of n-BuOH and ethylacetate (EtOAc) fractions were measured to be respectively 241.1 ± 1.1 and 222.9 ± 2.4 (mg/g GAE), and the total flavonoid content of EtOAc fraction was 75.3 ± 2.0 (mg/g QE). Upon anti-oxidant studies with DPPH and ABTS+ radicals, potent radicalscavenging activities were observed in the EtOAc and n-BuOH fractions. Moreover, in the study of cell protection efficacy usingHaCaT keratinocytes damaged by H2O2, the EtOAc and n-BuOH fractions showed a very positive results on prevention of oxidativestress. Phytochemical studies for this extract resulted in the isolation of four compounds; 2-oxopomolic acid (1), euscaphicacid (2), epi-catechin (3), prunasin (4). These results suggested that the extract of S. alnifolia branches containing compounds1-4 as natural ingredients could be used as whitening and anti-oxidant ingredients in cosmetic formulations.

[学科分类] 化学（综合）

[关键词] Sorbus alnifolia;Whitening;Anti-oxidation;Cell protective effect;Isolation