Evaluating the usefulness of liver biopsies in paediatric liver diseases

[摘要] Background: The involvement of surgical margins is a key factor determining the prognosis andmanagement of malignancies. Some specimens require inking up to six margins.Objectives: 1.To determine the best colours to ink margins using two freely available local paintbrands, Fabrica [FB] and Fevicryl [FV]. 2. To determine the best colour combinations to assess twoadjacent surgical margins.Method: Initially, the circumference of a total of 140 sections of lipomatous tissue, (1x1cm), werepainted with black (BK), blue (BL), brown (BR), green (GR), orange (OR), red (RE) and yellow (YE) usingFV and FB. Two investigators separately scored the colour retained [circumferential score 1-4 (1-25%1, 26-50%-2, 51-75%-3, 76%-100%-4)], the intensity and the original colour score. A composite gradewas obtained by the sum of the Z-scores of these three. Secondly, the four best colours, FV-BK, FVGR, FB-BL and FV-RE, were used to paint the circumference of 20 sections of lipomatous tissue (2x2cm) to get six combinations (BK-GR, BK-BL, BK-RE, GR-BL, GR-RE and RE-BL). The Z-scores were derivedfrom the differentiation scores given by two independent investigators. The intensity score, originalcolour score and differentiation scores (1-4) were graded as:1- barely visible X400, 2 - visible X400, 3- visible X100, 4 - visible X40. Two-way analysis of variance (ANOVA) and a post hoc mean comparison(Tukey HSD) were performed.Results: Higher standardized composite scores were obtained for FV compared to FB, except for BLand OR. FV-BK had the highest score, while FV-OR scored the lowest. The best colour combinationswere BK-GR, GR-RE and BL-RE, while GR-BL was the worst.Conclusion: Black, green, red, brown (Fevicryl) and black, blue, orange (Fabrica) were the seven bestcolours. The best combinations were black-green, green-red (Fevicryl), and blue (Fabrica) with red(Fevicryl).

[学科分类] 生理学与病理学