Purification and Characterization of a Detergent Compatible Alkaline Protease Produced by Bacillus ruris Isolated from Vegetable Oil Factory Effluent in Owo, Ondo State, Nigeria

[摘要] A thermostable alkaline protease was produced by Bacillus ruris isolated from vegetable oil factory effluent in Owo, Ondo State, Nigeria. Detergents containing enzymes have been reported to possess more effective washing capabilities. The protease was purified and characterized. Biochemical characterization and 16S rRNA sequencing showed the strain was closely related to Bacillus ruris with accession number NR_042161.1. The protease was purified with a 0.516 purification fold and 19% recovery with a Sephadex G-100 column fraction. The protease was relatively stable at alkaline pH retaining activity at pH 9.0 (100%). The protease was also thermally stable with the highest activity observed at 55 ℃. Bacillus ruris 2+ 2+ 2+ 2+ 2+ 2+protease activity was stimulated by Ba , Ca , Cu and Mn , while enzyme activity was inhibited by Zn and Pb . Protease activity increased with an increase in substrate concentration. The Lineweaver-burk plot revealed Km = 0.14. Protease activity was influenced by the tested surfactants and inhibitors. The protease enzyme showed relative stability to some commercial detergents. Thus, the protease enzyme appears to possess properties desired for detergent formulation and inclusion in other biotechnological applications.

[学科分类] 生物科学（综合）

[关键词] Protease;enzyme activity;detergents;alkaline;Bacillus ruris;purification.