Phytochemical profiling and in vitro α-amylase inhibitory activity of Glycosmis pentaphylla (Retz.) DC.

[摘要] The present work has been done with an objective to analyze the phytochemical composition responsible for plausible antidiabetic effect of leaf, stem and root of Glycosmis pentaphylla (Retz.) DC. Fresh leaf, stem and root of Glycosmis pentaphylla were extracted with ethanol (EEGPle, EEGPst, EEGPro) and evaluated by phytochemical analysis for their infinite primary and secondary metabolites viz. carbohydrates, proteins, alkaloids, flavonoids, terpenoids, glycosides, saponin and phenols. The antiabetic effect was done with the help of alpha amylase inhibitory assay. Estimation of total flavonoid content (TFC) and total phenolic content (TPC) was also done to confirm the presence of these phytochemicals. The phytochemical analysis of EEGPle revealed that carbohydrates, proteins, alkaloids, flavonoids, glycosides, tannins, phenols and saponins were present, whereas, EEGPst showed the presence of proteins, alkaloids, flavonoids, phenols and terpenoids. Presence of carbohydrates, alkaloids, phenol and terpenoids were found in EEGPro. A significant hypoglycemic activity was revealed by the EEGPle [EC50: (23.14±0.006) µg/mL], compared to EEGPst and EEGPro. Quantity of TFC and TPC was highest in EEGPle (112.96±3.89 mg QRE/g and 96.6±1.08 mg GAE/g extract) rather than EEGPst and EEGPro extracts. The present work suggests that EEGPle has a significantly higher anti-diabetic property than EEGPst and EEGPro. These extracts can help in preventing or slowing down the occurance of diabetes, but a detailed analysis of these extracts is required to determine the presence of promising compound(s) responsible for their anti-diabetic potential.

[学科分类] 生物科学（综合）

[关键词] Glycosmis pentaphylla;Phytochemicals;Anti-diabetic activity;Hyperglycemic