AFLP Primer Selection for the Analysis of Genetic Diversity in Persimmon (Diospyros kaki L.) Originated From Central and East Java, Indonesia

[摘要] Persimmon (Diospyros kaki L.) belongs to the family Ebenaceae known as the Japanese persimmon kaki. This crop was introduced in the Highlands in Central and East Java-Indonesia. A genetic analysis of a small sample of accessions was conducted using the Amplified Fragment Length Polymorphism (AFLP) method with IRDye700 labelled Pst1 (P11-700) and Mse1 restriction enzymes and seven primers combinations (M48, M49, M50, M51, M53, M55, and M58). The analysis resulted in a set of 441 bands, of which 117 were monomorphic and 324 polymorphic. The average percentage of polymorphic bands was 73.4%. The four persimmon accessions were genetically distinguished into three groups, with a high genetic diversity among them, while accessions B (Batu) and D (Dampit) show little differences in their profiles.

[学科分类] 生物科学（综合）

[关键词] Diospyros kaki L.;AFLP markers;Pst1;Mse1;genetic diversity.