Effect of Garlic, Vitamin C, Vitamin E–Selenium against Bioaccumulated Organolead-Induced Cellular Injury in Liver and Spleen of Albino Rats: Pilot Study

[摘要] 2+Exposure to Lead (Pb ) in its organic and inorganic forms is known to have deleterious effects on the health of individuals. Several studies showed that these effects are due to accumulation in vital organs and induction of oxidative stress. This study examines the possibility of three different antioxidant substances (Aqueous garlic extract, Vitamin C and Vitamin E – Selenium) to act as chelating agents against organolead toxicity (lead acetate) by decreasing its accumulation in liver and spleen. To achieve this purpose, forty-eight adult male albino rats were divided into eight equal groups; Group I: Control received normal water, Group II: received lead acetate (100 mg/kg/day; i.p), Group III: received garlic (100 mg/kg; orally), Group IV: received Lead (100 mg/kg/day; i.p) + Garlic (100 mg/kg; orally), Group V: received Vitamin C (100 mg/kg; orally), Group VI: received Lead (100 mg/kg/day; i.p) + Vitamin C (100 mg/kg; orally). Group VII: received Vitamin E (100 mg/kg; orally) + Selenium (0.5 mg/kg; orally) and Group VIII: received Lead (100 mg/kg/day; i.p) + Vitamin E (100 mg/kg; orally) + Selenium (0.5 mg/kg; orally). The experiments were performed over four consecutive weeks, after which blood was withdrawn from rats by heart puncture and animals were sacrificed by cervical dislocation; the liver and spleen were removed to quantify their lead content by Flame Atomic Absorption Spectroscopy (FAAS). Analysis of the serum showed that lead acetate has significantly elevated the activity of the ALT, AST, and LDH compared to the control group, and the three selected antioxidant substances were able to minimize the activity of these enzymes significantly in comparison with lead acetate group. In addition, the results from the FAAS showed that lead concentration has significantly increased in the liver and spleen of the lead acetate group compared to the control group, and that treatment with antioxidants was not effective in reducing that effect. It is concluded that the selected antioxidants had an ameliorative effect against the hepatotoxicity induced by lead acetate but could not be considered the suitable heavy metal chelator to overcome the bioaccumulated lead in the vital organs.

[学科分类] 生物科学（综合）

[关键词] Antioxidants;Bioaccumulation;Biochemical Markers;Lead Acetate;Metal Chelators