Antioxidant effect of Beta-D-glucan-polysaccharide fractionate of Auricularia polytricha on Hyperglycaemia-Induced Kidney Dysfunction in Experimental Diabetic Nephropathy

[摘要] β-D-Glucan polysaccharide is a major bioactive component fractionated from Auricularia polytricha (mushroom), an edible fungus used for both nutritional and therapeutic purposes. Local Nigerian men use A. polytricha to manage diabetes related complications without accepted experimentation to ascertain the role and mechanism of action of it fractionates. This study was intended to find out the effect of β-D-Glucan polysaccharide supplementation of hyperglycaemia-induces kidney dysfunction in diabetic Wistar rat. Experiment animals were divided into four groups and received treatment as follows; Group A (treated with normal saline and served as normal control), Group B, (diabetic control animals were treated with 65mg/kg.bw of streptozotocin (STZ)), Groups C and D, (placed on 120 mg/kg.bw and 200 mg/kg.bw of β-D-Glucan polysaccharide respectively after inducing diabetics). At termination, 24 hours urine was collected and analyzed for creatinine clearance and proteinuria, both kidneys were harvested and weighed, serum was collected by cardiac puncture and analysed for serum creatinine and oxidative stress markers (Superoxide Dismutase. Catalase and Malondialdehyde), while histopathological examination was done using routine Hematoxyline and Eosine technique with the help of light microscope. The result showed that hyperglycaemic ambience caused a significant increase in serum level of oxidative stress markers along with concomitant cytoarchitectural alterations of kidney tissue and a change in values of renal parameters (serum creatinine, proteinuria, and creatinine clearance) indicating kidney dysfunction in diabetic control animals. However, decrease in activities of oxidative stress markers, reversal of alterations in kidney microstructure, and the overall improvement in values of renal parameters were observed following administration of β-D-Glucan polysaccharide in a dose-dependent manner. The present study suggests that β-D-Glucan polysaccharide supplementation can ameliorate hyperglycaemia-induced nephrotoxicity in diabetic wistar rat model.

[学科分类] 生物科学（综合）

[关键词] β-D-Glucan polysaccharide;diabetic nephropathy;Auricularia polytricha;kidney;oxidative stress