A Review on Reliability and Validity of CRISPR/Cas9 Technology for Gene Editing

[摘要] Genome engineering is one of the worldwide fast growing field of biotechnology which involves designed programmable DNA-binding nucleases such as homing endonucleases, zinc finger nucleases (ZFNs), transcriptions activator like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated 9) nucleases. These technologies utilize manipulated nucleases which are the complex of sequence-specific DNA binding domains and nonspecific DNA cleavage modules. CRISPR)/Cas9 technology lets scientists accurately cut and paste genes into DNA which can be applied to edit the individual gene or even entire chromosomes from an organism at any point in its development, become a magical tool due to its simplicity. Here we review the four basic pieces of information on the genome editing technologies with their reliability and discuss the applications and their therapeutic potential as well as future prospects.

[学科分类] 生物科学（综合）

[关键词] Gene editing;CRISPR/Cas9 technology;endonucleases mechanism;transcription.