Green Synthesis of Silver Nanoparticles using Neem and Collagen of Fish Scales as a Reducing and Stabilizer Agents

[摘要] This study was conducted to synthesize and characterize silver nanoparticles (AgNPs) using a rapid green synthesis approach. Antibacterial properties of AgNPs were evaluated. Extract of Melia Dubia (Neem) and collagen produced from the fish scales were used as reducing and stabilizer agents respectively. Uv-Vis Spectroscopy, Scanning Electron Microscopy (SEM), Energy-Dispersive X-Ray Spectroscopy (EDX), Fourier-Transform Infrared Spectroscopy (FTIR), and Dynamic Light Scattering (DLS) were used to characterize the synthesized AgNPs. Evaluation of the synthesized AgNP’s antibacterial activity was conducted, with Staphylococcus Aureus (S. aureus) as Gram-positive and Escherichia Coli (E. coli) as the negative bacteria. The peak of absorbance for the synthesized AgNPs was at 454 nm, indicating conformed AgNPs. SEM image showed semi-evenly distributed rod shapes. The EDX data revealed presence of the metallic silver. Meanwhile, FTIR analysis indicated presence of C2H2, C=O, N-H groups. DLS showed an average size of 437.6 nm. XRD showed calculation for particle size using d = Kλ/βcosθ. Average size of AgNPs was 141.81 ± 5 nm. AgNPs also displayed tangible antibacterial activity towards the S. aureus and pathogenic E. coli. AgNPS were successfully synthesized and evaluated for their antibacterial properties. The outcomes being the multifaceted biological activities alongside application of biocompatible green nanoparticles, is discoverable in the field of Nanomedicine.

[学科分类] 生物科学（综合）

[关键词] Silver nanoparticles;Fish Scales Collagen (FsCol);Melia Dubia (neem);Antibacterial