Metabolites profiling of Limonium tubiflorum (Delile) Kuntze var tubiflorum via UPLC-qTOF-MS technique in relation to its cytotoxic activity

[摘要] Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) technique led to the detection of 55 metabolites in Limonium tubiflorum (Delile) Kuntze var tubiflorum flowers extract for the first time including 38 flavonoids, 7 phenolic acids and 10 anthocyanins. The total phenolic content was (231.225 mg -1 -1GAE g DW ) and the total flavonoid content was (136.66 mg rutin g DW ) in L. tubiflorum (Delile) Kuntze var tubiflorum. The flower plant powder has been prepared by successive fractionation. The successive extracts have been tested for in vitro cytotoxic activity against MCF7, HEPG2, and HCT116 and exhibited moderate to weak cytotoxic activities compared to Doxorubicin (positive control) which showed in vitro cytotoxic effect with IC values of 2.97 ± 0.9, 4.57 ± 0.5 and 3.73 ± 500.6 µg/mL, respectively. The results also showed that the 95% ethanolic successive extract exhibited the strongest cytotoxic activity among all the tested extracts against HEPG2 and HCT116 cell lines with IC values of 21 ± 0.5 and 21 ± 0.8 50µg/mL, respectively, while 70% ethanolic successive extract showed the highest cytotoxic activity against MCF7 cell line with IC value (26.6 ± 0.7 µg/mL). The hydro-ethanolic plant flower extracts are enriched in phenolic compounds 50(flavonoids, phenolic acids and anthocyanins) which are likely to mediate for cytotoxic activity.

[学科分类] 生物科学（综合）

[关键词] Limonium tubiflorum (Delile) Kuntze var tubiflorum;UPLC-qTOF-MS;flavonoids;anthocyanins;cytotoxicity