Employment of Somatic Embryogenesis as a tool for Rescuing Imperiled Narcissus tazetta L. Growing wild in Jordanian Environment.

[摘要] Somatic embryogenesis was used as a tool for micropropagation of wild Narcissus tazetta plants exposing to overcollection and rapidly changed environmental conditions. In the growth regulators experiment, somatic embryogenesis was successfully induced in all treatments except for explants grown in the control treatment (Murashig and Skoog (MS) hormone-free medium). Meanwhile, the highest value for the number of somatic embryos/ callus segment (441) was -1obtained into MS media supplemented with 0.2 mg l 6-(gamma, gamma- Dimethylallylamino) purine (2iP) under dark -1 conditions. Moreover, sucrose at 30g L was the best sugar source resulting in higher number of somatic embryos compared to the other sugar treatments. The highest shoot development rate from somatic embryos was (191.99/ non-embryonic calli -1segment) recorded in cultures grown on MS media plus 0.5 mg l 2iP. Maximum bulbet size (1.6 cm diameter) was recorded in plantlets kept onto hormone-free MS hormone free media for 6 weeks before acclimatization, while less durations resulted in smaller bulbet size. Well developed in vitro plantlets were acclimatized successfully with high survival percentage of (95%). The acclimatized plants were normal and did not show any morphological abnormalities.

[学科分类] 生物科学（综合）

[关键词] Callus;Embryogenesis;Narcissus tazetta;Somatic embryo.