Diode Laser Irradiation Effects on Miniscrew Stability and IL-1β and TGF-β1 Levels: A Split-Mouth Randomized Controlled Clinical Trial

[摘要] Introduction: This study aimed to assess the effect of low-level laser therapy (LLLT) on miniscrewstability and concentrations of interleukin-1β (IL-1β) and transforming growth factor-beta (TGF-β1)in peri-miniscrew crevicular fluid in the course of orthodontic treatment.Methods: This randomized split-mouth double-blind clinical trial evaluated 18 patients requiringanterior retraction along with maximum anchorage. Miniscrews were placed between the maxillarysecond premolar and first molar. A diode laser was irradiated with a 980-nm wavelength and100-mW output power in continuous-wave mode at four time points: T0 (1 hour after miniscrewplacement), T1 (1 week later), T2 (at 1 month) and T3 (at 3 months) in one quadrant of the maxilla(laser group). The other quadrant of the maxilla underwent the pseudo-application of the laser(control group). The primary stability of miniscrews was measured by Periotest M and reported asPeriotest value (PTV). Also, at each time point, samples were collected from the peri-miniscrewcrevicular fluid one hour after laser irradiation to assess the concentration of IL-1β and TGF-β1.Results: The mean PTV (inverse of the stability) was smaller in the laser group compared with thecontrol group at all time points; this difference was significant at T2 and T3. The mean concentrationof IL-1β in the laser group was lower than that in the control group at all time points, and thisdifference was significantly remarkable at T0 and T3. The mean concentration of TGF-β1 in thelaser group was lower than that in the control group at T0, T1 and T3; however, the difference wasnot statistically significant.Conclusion: The current results supported the efficacy of LLLT in increasing the miniscrew stabilityand decreasing the level of IL-1β pro-inflammatory cytokine.

[学科分类] 基础医学

[关键词] Miniscrew stability;Low-level laser therapy;Interleukin-1β;Transforming growthfactor-β1.