Effect of Er: YAG Laser Irradiation on Bone Metabolism-Related Factors Using Cultured Human Osteoblasts

[摘要] Introduction: A variety of laser treatments have been applied in numerous medical fields. Indentistry, laser treatments are used for caries, root canals, and periodontal disease, as well assurgical resection. Numerous reports have recently been published on the use of lasers for boneregeneration. If laser irradiation is found to promote the activation of bone metabolism, it mightalso be effective for periodontal treatment, peri-implantitis, and bone regeneration. Therefore, thepresent in vitro study aimed to elucidate the mechanisms underlying the effects of erbium-dopedyttrium aluminum garnet (Er: YAG) laser irradiation on the bone using osteoblast-like cells.Methods: Osteoblast-like Saos 2 cells (5.0×104 cells) were seeded in 24-well plates. 24 hours afterbeing seeded, the cells were subjected to 0.3 W, 0.6 W, and 2.0 W Er: YAG laser irradiation and thenallowed to recover for 48 hours. The expression levels of bone metabolism-related factors alkalinephosphatase (ALP), bone sialoprotein (BSP), and osteoprotegerin (OPG) were then evaluated usingreverse transcription–quantitative polymerase chain reaction and western blot analyses.Results: Saos 2 cells subjected to Er: YAG laser irradiation at 0.3 W, 0.6 W, and 2.0 W showednormal growth. When the Er: YAG laser irradiation and control groups were compared after 48hours, increases were observed in ALP, BSP, and OPG gene and protein expression in the 2.0 Wgroup. Similar results were obtained in the western blot analysis.Conclusion: These findings suggest that the Er: YAG laser irradiation of osteoblast-like cells iseffective for activating bone metabolism factors.

[学科分类] 基础医学

[关键词] Bone metabolism;Bone restoration;Er: YAG laser;Osteoblasts