In Vitro Efficacy of Antimicrobial Photodynamic Therapy With Phycocyanin and Diode Laser for the Reduction of Porphyromonas gingivalis

[摘要] Objectives: This study sought to assess the efficacy of antimicrobial photodynamic therapy (aPDT) with phycocyanin and diode laser for reduction of Porphyromonas gingivalis (P. gingivalis). Materials and Methods: In this in vitro, experimental study, first the minimum inhibitory concentration (MIC) of phycocyanin, the sub-lethal exposure time of diode laser, and the sub-lethal dose of aPDT were determined. The wells containing P. gingivalis suspension were randomly divided into three experimental groups for exposure to ½ MIC phycocyanin for 5 min, minimum lethal dose of diode laser (635 nm, 4 min), and aPDT with phycocyanin and diode laser. The positive control group was exposed to 0.2% chlorhexidine (CHX) for 5 min, and the negative control group received no treatment. The colony count was calculated in the five groups and compared using one-way ANOVA and t-test. Results: aPDT with diode laser (635 nm, 4 min) and 125 µg/mL phycocyanin caused significantly greater reduction of P. gingivalis count (mean reduction of 44.24%) compared with other groups (P<0.0001). Minimum and maximum colony counts were noted in 0.2% CHX and negative control groups, respectively. The reduction in colony count was significant in all experimental groups, compared with the control group (P<0.0001). Conclusion: aPDT with 635 nm diode laser and phycocyanin can significantly decrease P. gingivalis count in vitro. Considering the conservative nature of this modality, it may be used for decontamination of peri-implant.

[学科分类] 基础医学

[关键词] Photochemotherapy;Phycocyanin;Porphyromonas gingivalis;Antimicrobial photodynamic therapy;Peri-implant