Wound Healing Potency Of Bacterial Cellulose Membranes Loaded With Different Antibiotics.

[摘要] Bacterial cellulose (BC) is a natural polymer used in wound healing. It was obtained fromKomagataeibacter hansenii. Gentamicin, ampicillin and chloramphenicol at different concentrations wereloaded into BC membranes. BC membranes loaded with different antibiotics were chemically characterized andinvestigated for their antibacterial and wound healing properties. The results elucidated high water holdingcapacity of BC membrane. In addition, the antibiotics loading capacities of BC membranes at differentconcentrations were higher in gentamicin followed by ampicillin then chloramphenicol. In vitro antibacterialeffects were carried out against some Gram-negative and Gram-positive bacterial strains. BC-Chloramphenicolmembrane was active against all used bacterial models. BC-Ampicillin membrane showed activity against allmodels of bacteria except Staphylococcus aureus, while BC- gentamicin membrane was active againstPseudomonas aeruginosa and Bacillus subtilis. BC and BC membranes loaded with different antibiotics wereinvestigated for their wound healing potency in wounded rat model for 14 days. These membranes reduced theinflammation through regulating the expression of intercellular adhesion molecule 1, tumor necrosis factor -αand interleukin-6.This was followed by enhancing wound tissue collagen I, hydroxyproline, total protein,fibronectin and vascular endothelial growth factor with a reduction in tissue myeloperoxidase so they can beused as perfect dressing materials.

[学科分类] 工程和技术（综合）

[关键词] Bacterial cellulose;Antibacterial;Wound healing.