In-vitro Antiglycation Activity of Zinc Oxide Nanoparticles Synthesized from the Bioactive Fraction of Bambusa arundinacea Leaf Extract.

[摘要] The present study was conducted to investigate an invitro antiglycation capacity of biologicallysynthesized zinc oxide nanoparticles (ZnO NPs) from zinc acetate and sodium hydroxide utilizing anantioxidant-rich fraction of Bambusa arundinacea leaf extract. ZnO NPs were characterized by FTIR, TEM andUV–Vis spectroscopy. The antiglycation potential of ZnO NPs was measure by using the formation of AGEsfluorescence intensity and the level of fructosamine in bovine serum albumin (BSA)-glucose assay. Theinhibitory activity of glycosylated Hb was also measured. Four fractions were collected using columnchromatography of Bambusa arundinacea leaf extract named F1, F2, F3 and F4. The results showed that F3exhibits the greatest phenolic content (87.36 ± 9.32 mg gallic acid equivalent (GAE)/g) and flavonoid content(29.65 ± 6.52 mg quercetin equivalent (QE)/g). In the DPPH radical scavenging activity, F3 had the lowestIC50 values of 46.7 µg/ml indicate the highest antioxidant potential. Based on this observation, F3 was used asa reducing agent for the synthesis of ZnO NPs. The Result of TEM showed that ZnO NPs were spherical in shapewith a size range of 60-98 nm. The result showed that ZnO NPs at different time duration (1-4 weeks) havesignificantly inhibited the formation of AGEs in terms of the fluorescence intensity of glycated BSA of thestudy. The ZnO NPs also markedly declined the level of fructosamine and formation of glycosylated Hb whichare directly associated with the reduction of advanced glycation end products (AGEs) formation. Overall, thestudy suggests biologically synthesized zinc nanoparticles showed the strong antiglycation potential andconsidered as a potential source of therapeutic agents for AGEs related disorder.

[学科分类] 工程和技术（综合）

[关键词] Antiglycation;Bambusa arundinacea;Nanoparticles;Zinc oxide