Antimicrobial, Anti-Inflammatory Effects Of Centella asiatica Leaf And Their Cytotoxic Effects On MCF-7 And HeLa Cell Lines.

[摘要] Centella asiatica leaf contains a vast range of phytochemicals which proven with its medicinalproperties. In present study, ethanol extract of Centella asiatica was studied for its phytochemical andpharmacological properties. C. asiatica extracts indicates the presence of Asiatic acid, Asiaticoside,Madecassoside and Madecassic acid. The extract containing these valuable compounds were tested againstmicroorganisms using a disc diffusion method. It was observed that the extract showed greater bactericidal ongram negative bacteria compared with gram positive bacteria and there was antifungal activity towardsAspergillus niger. For anti-inflammatory test, the highest concentration of extract (300mg/ml) gave the highestdenaturation percentage of 51%. The MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay was usedto detect the viability of the cells when tested with Centella asiatica leaf extract at various concentration basedon the formazan production from the bromide. Besides that, 200µl Centella asiatica leaf extract with aconcentration of 100mg/ml exhibited anticancer activity on MCF-7 and HeLa cells. The findings from this studyindicated that Centella asiatica leaf extract can be used in the pharmaceutical industry as an antimicrobial, anti- inflammatory and anticancer drug.

[学科分类] 工程和技术（综合）

[关键词] Centella asiatica;Antimicrobial;Anti-Inflammatory;Cytotoxicity